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Карта сайта Интеллектуальная поисковая системаДобавить свою ссылку по ключевому запросуПоисковая система v3.kz поможет ускорить индексацию вашего сайта выводит в Топ |
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Dharmacon™ RNAi, Gene Expression & Gene Editing Protocol DharmaFECT™ Transfection Reagents siRNA Transfection Protocol The following is a general protocol for use of Dharmacon DharmaFECT transfection reagents to deliver siRNA into cultured mammalian cells. An example protocol of siRNA knockdown is explained here using the siGENOME SMARTpool reagents from Dharmacon. Dharmacon™ RNAi, Gene Expression Gene Editing Protocol GE Healthcare DharmaFECT™ Transfection Reagents siRNA Transfection Protocol The following … (800) 235-9880 (toll-free, in the U.S.) or (303) 6049499, or e-mail us at the addresses below: United States techsupport@dharmacon.com www.dharmacon.com Europe/Israel Perbio Science eurotech@perbio.com www.perbio.com DharmaFECT General Transfection Protocol... Just go back to the protocol of Dharmacon siRNA and follow them strictly, you should easily get a nearly 100% mRNA konckdown efficiency at 48~72hrs post transfection. DharmaFECT® Duo Transfection Reagent Protocol 00135-06-J-01-U Page 2 of 2 Korea SeouLin Bioscience sltrade@seoulin.co.kr www.seoulin.co.kr Taiwan Tseng Hsiang Life Science, Ltd. info@thco.com.tw www.thco.com.tw Other techsupport@dharmacon.com. The Bottom Line. Reagent with high transfection efficiency in a broad range of cell lines, Dharmacon has optimized protocols (available at the companys website) for a variety of cell lines. Product Insert Thermo Scientific Dharmacon® DharmaFECT® Duo Transfection Reagent – for siRNA and Plasmid Co-Transfection Low transfection efficiency Follow transfection protocol steps carefully. To assess transfection efficiency of siRNA use Mirus Bio’s Label IT sirna transfection using fugene siglo red transfection indicator dharmacon hela monster transfection reagent mirus roche fugene 6 transfection protocol. 293t cells using lipofectamine. Информация взята v3.kz |
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